Genetic Transformation of Murine Bone Marrow Cells

نویسندگان

  • William D. Medina
  • Shyam Dube
  • Joseph R. Bertino
چکیده

Genetic transformation of murine bone marrow stem cells to methotrexate resistance was achieved using a modified calcium phosphate-DNA coprecipitation procedure. Bone marrow cells were transformed by DNA derived from methotrexate-resistant mouse 3T6 cells. In vivo selection of drug-resistant bone marrow cells resulted from thrice weekly injections of methotrexate (MTX) for a period of 6-8 wk. Following selection. dihydrofolate reductase activity encoded by the donor DNA species was easily detectable in extracts of recipient mouse spleens. In addition.

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تاریخ انتشار 2005